| 摘要 |
Recombinant Corynebacterium DNA (I), present in microorganisms of the Corynebacterium genus and comprising at least one of the panB, panC and or ilvD genes, is new. Recombinant Corynebacterium DNA (I), present in microorganisms of the Corynebacterium genus and comprising at least one of the (i) panB (ketopantohydroxymethyltransferase), (ii) panC (pantothenicacidsynthetase), especially the panBC operon, and/or (iii) ilvD (dihydroxyaciddehydratase) genes, and comprising the fully defined 2164, 2164 and/or 2952 base pair sequences, respectively, is new. Independent claims are also included for the following: (1) microorganisms (II), especially Corynebacterium, transformed with one or more of (I); (2) shuttle vector pECM3ilvBNCD (III), deposited as E. coli DH5 alpha mcr/pECM3ilvBNCD under DSM 12457; (3) shuttle vector pEKEx2panBC (IV), deposited as E. coli DH5 alpha mcr/pEKEx3panBC under DSM 12456; and (4) a method (V) for the preparation of pantothenic acid. |
