METHOD FOR PREPARING (R)- OR (S)-N-(2,6-DIMETHYL PHENYL) ALANINE AND STEREOISOMERS OF CORRESPONDING ESTER THERETO USING ENZYME

摘要

PURPOSE: A method for preparing (R)- or (S)-N-(2,6-dimethyl phenyl) alanine and stereoisomers of corresponding ester thereto using enzyme are provided, thereby cheaply and simply conducting the optical resolution of racemic (R), (S)-N-(2,6-dimethyl phenyl) alanine ester to prepare (R)- or (S)-N-(2,6-dimethyl phenyl) alanine having antimicrobial activity, and ester thereto in higher yield. CONSTITUTION: The method for preparing (R)- or (S)-N-(2,6-dimethyl phenyl) alanine and stereoisomers of corresponding ester thereto using enzyme comprises the steps of: (A) reacting racemic (R), (S)-N-(2,6-dimethyl phenyl) alanine ester of formula (1) with enzyme which specifically hydrolyzes (R)- or (S)- form of optical isomers of racemic (R), (S)-N-(2,6-dimethyl phenyl) alanine ester to prepare (R)- or (S)-N-(2,6-dimethyl phenyl) alanine; and (B) separating the (R)- or (S)-N-(2,6-dimethyl phenyl) alanine from unreacted corresponding (R)- or (S)-N-(2,6-dimethyl phenyl) alanine ester, wherein R is selected from optionally substituted C1-18 linear or branched chain alkyl or alkenyl, optionally substituted C3-6 cycloalkyl, optionally substituted aryl alkyl, and optionally substituted heteroaryl alkyl.