| 摘要 |
Disclosed are protease variants including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 and at one or more of positions 236 and 245 of Bacillus amyloliquefaciens subtilisin and substituting one or more amino acids at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 16-22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75-79, 86, 87, 89, 97-99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140-142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203-206, 209-218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242-244, 246-249, 251, 252-263, 265, 268-272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when a substitution of amino acid residues at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin. Such variants are useful in cleaning compositions, compositions for treating textiles and animal feeds. (62) Divided out of 503909 |
