| 摘要 |
A method of tranducing the conformational change of a signaling aptamer that occurs upon the signaling aptamer binding a ligand to a differential optical signal generated by a reporter molecule comprises the steps of: covalently coupling the reporter molecule to an aptamer to form the signaling aptamer wherein the reporter molecule replaces a nucleic acid residue in the aptamer or is inserted between two nucleic acid residues in the aptamer such that the placement does not interfere with the ligand-binding site of the aptamer; contacting the signaling aptamer with the ligand under conditions whereby the signaling aptamer binds the ligand; and detecting the differential optical signal generated by the reporter molecule transduced by the conformational change in the signaling aptamer upon binding the ligand. Preferably the optical signal is selected from fluorescence, colorimetric intensity, anisotropy, polarization, lifetime emission wavelength and excitation wavelength and the reporter molecule is a fluorescent dye.
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