N-Acetyl-D-Glucosamin Deacetylase und Verfahren zu deren Herstellung

摘要

There are provided N-acetyl-D-glucosamine deacetylase capable of hydrolyzing acetamide group of N-acetyl-D-glucosamine to produce D-glucosamine and acetic acid, which has the following physicochemical properties: (1) Substrate specificity: N-acetyl-D-glucosamine monomer, not the oligomer or polymer thereof; (2) Optimal pH: 7.8-8.2 at 37 DEG C; (3) Stable pH: 6.0-9.0 at 45 DEG C; (4) Optimal temperature: 28-39 DEG C; (5) Molecular weight: 70,000-200,000 as determined using TSK-gel G-3000 SW column, and a process for preparing the same using a microorganism belonging to Vibrio sp.