发明名称 |
Method for determining polynucleotide sequence variations |
摘要 |
A method of determining the presence and identity of a variation in a nucleotide sequence between a first polynucleotide and a second polynucleotide, comprising a) providing a sample of the first polynucleotide; b) selecting a region of the first polynucleotide potentially containing the variation; c) subjecting the selected region to a template producing amplification reaction to produce a first plurality of double stranded polynucleotide templates which include the selected region; d) selecting a region of the first polynucleotide sequence lying within the templates for analysis; e) producing a family of labeled, linear polynucleotide fragments from both strands of the templates simultaneously by a fragment producing reaction.
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申请公布号 |
US2004121394(A1) |
申请公布日期 |
2004.06.24 |
申请号 |
US20030733931 |
申请日期 |
2003.12.10 |
申请人 |
DAWSON ELLIOTT P.;PHILLIPS JOHN A. |
发明人 |
DAWSON ELLIOTT P.;PHILLIPS JOHN A. |
分类号 |
C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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