| 摘要 |
The present invention utilizes nucleic acid fusion proteins as secondary binding agents for detecting protein-protein interactions. The methods involve binding a target protein with a binding agent that is a protein nucleic acid fusion protein, wherein each binding agent has a unique nucleic acid sequence. Once binding of a fusion protein to a target protein occurs, the nucleic acid portion of the fusion protein is amplified and used to probe an array of nucleic acid molecules that are complementary to the amplified nucleic acid portions of the fusion proteins. Detection of the hybridized nucleic acid portion of the array identifies and quantifies the protein-protein interaction.
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