HIGH EFFICACY ANTISENSE Rialpha PKA POLY-DNP OLIGORIBONUCLEOTIDES
摘要
The present invention discloses antisense poly-2'-O-(2,4-dinitrophenyl) oligoribonucleotides which are capable of down regulating the expression of the RIalpha subunit of protein kinase A. An example is 5'-GGCUGCGUGCCUCCUCACUGG (named antisense poly-DNP RNA21) or a sequence which has a one-base mismatch therewith. The antisense oligoribonucleotide can be synthesized by in vitro transcription followed by chemical derivatization. The base sequence of the oligoribonucleotides is complementary to that of nt 110 to 130 in RIalpha/PKA mRNA. The antisense poly-DNPRNA-21 was found to inhibit cell growth with IC50 values in the nanomolar range. These oligonucleotides can be used as effective anti-cancer agents.
申请公布号
WO2004053073(A2)
申请公布日期
2004.06.24
申请号
WO2003US38673
申请日期
2003.12.05
申请人
THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEWYORK;WANG, JUI, H.;SHEN, LONG;CHEN, XIAOLAN