摘要 |
According to the invention, the desoxyribonuclease with increased thermolability is a variant, by way of amino acid substitution, of bovine pancreatic desoxyribonuclease I. The variant protein retains desoxyribonuclease activity. Moreover, the specific desoxyribonuclease activity of the variant of bovine pancreatic desoxyribonuclease I is approximately zero units per mg of protein following heating of the variant of bovine pancreatic desoxyribonuclease I for about 5 min at a temperature below 95 DEG C. In addition, the variant of bovine pancreatic desoxyribonuclease I has no measurable ribonuclease activity.
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申请人 |
ROCHE DIAGNOSTICS GMBH;F. HOFFMANN-LA ROCHE AG |
发明人 |
MUELLER, RAINER, DR.;KIRSCHBAUM, THOMAS, DR.;SUPPMANN, BERNHARD, DR.;SCHOEN, HELMUT, DR.;ENGH, RICHARD, DR.;HOFFMANN, ARTUR, DR.;THALHOFER, JOHAN-PETER, DR.;SIEDEL, JOACHIM, DR.;ENGEL, WOLF-DIETER, DR. |