REVERSE TRANSCRIPTASE FROM RETICULOENDOTHELIOSIS VIRUS BEING USEFUL FOR DIAGNOSIS OF DISEASE OR GENE CLONING AND MASS-PRODUCTION METHOD THEREOF

摘要

PURPOSE: Reverse transcriptase from Reticuloendotheliosis virus and a mass-production method thereof are provided. The enzyme is useful for diagnosis of disease or gene cloning by synthesis of cDNA from RNA. CONSTITUTION: The reverse transcriptase from Reticuloendotheliosis virus has the amino acid sequence set forth in SEQ ID NO:2. A gene encoding the reverse transcriptase from Reticuloendotheliosis virus has the nucleotide sequence set forth in SEQ ID NO:1. The method for mass-producing the reverse transcriptase from Reticuloendotheliosis virus comprises the steps of: constructing a vector containing the reverse transcriptase gene of SEQ ID NO:1; transforming Escherichia coli with the vector to produce a transformed E. coli; culturing the transformed E. coli in a medium; isolating cultured cells containing the expressed recombinant Reticuloendotheliosis virus reverse transcriptase; dissolving the isolated cells in a denaturation buffer solution containing urea or guanidine chloride; and reacting the dissolved protein with a renaturation buffer solution to refold the protein.