PEPTIDE MPI WITHOUT SIGNAL SEQUENCE, DNA ENCODING THEREOF, VECTOR, METHOD FOR ASSAY CAPACITY FOR SECRETION OF TESTING PEPTIDE, METHOD FOR ISOLATING CDNA

摘要

FIELD: molecular biology, biotechnology, genetic engineering. ^ SUBSTANCE: invention can be used for detection and isolation of DNA encoding peptide that elicits the secretory activity. DNA encoding human protein mpI without signal sequence is ligated in vector with testing cDNA encoding this peptide. Chimeric gene is expressed in cells for evaluating the capacity for cellular proliferation. The presence of signal peptide in testing cDNA is assayed by capacity for proliferation. DNAs encoding secretory proteins are detected by construction of cDNA library and the following its screening. Invention provides detection of secretory proteins, among them, membrane proteins of the I-st and the II-d type. ^ EFFECT: improved assay and isolating methods. ^ 11 cl, 1 dwg, 5 tbl, 3 ex