METHOD OF DETECTING TARGET BASE SEQUENCE OF RNA INTERFERENCE, METHOD OF DESIGNING POLYNUCLEOTIDE BASE SEQUENCE CAUSING RNA INTERFERENCE, METHOD OF CONSTRUCTING DOUBLE-STRANDED POLYNUCLEOTIDE, METHOD OF REGULATING GENE EXPRESSION, BASE SEQUENCE PROCESSING APPARATUS, PROGRAM FOR RUNNING BASE SEQUENCE PROCESSING METHOD ON COMP

摘要

In the present invention, a sequence segment conforming to the following rules (a) to (d) is searched from the base sequences of a target gene of RNA interference and, based on the search results, siRNA capable of causing RNAi is designed, synthesized, etc.: (a) The 3' end base is adenine, thymine, or uracil, (b) The 5' end base is guanine or cytosine, (c) A 7-base sequence from the 3' end is rich in one or more types of bases selected from the group consisting of adenine, thymine, and uracil, and (d) The number of bases is within a range that allows RNA interference to occur without causing cytotoxicity.