发明名称 |
Methods of protein purification and recovery |
摘要 |
Improved methods for purification and recovery of interferon-beta (IFN-beta) and compositions comprising substantially monomeric IFN-beta are provided. In one purification method, substantially purified IFN-beta or variant thereof is precipitated and then dissolved in a guanidine hydrochloride (HCl) solution. Renaturation of the protein occurs by dilution with a suitable buffer. A similar purification method absent the precipitation step is also provided. Following renaturation of the IFN-beta, residual guanidine HCl is removed by diafiltration or dialysis with a pharmaceutically acceptable buffer to prepare pharmaceutical compositions comprising substantially monomeric IFN-beta.
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申请公布号 |
US2004115169(A1) |
申请公布日期 |
2004.06.17 |
申请号 |
US20030750076 |
申请日期 |
2003.12.31 |
申请人 |
CHIRON CORPORATION |
发明人 |
WOLFE SIDNEY N.;SHIRLEY BRET A.;BABUKA SUSAN;FORDHAM DENNIS;ESIKOVA IRINA |
分类号 |
A61K38/21;C07K14/565;(IPC1-7):A61K38/21;C07K14/56 |
主分类号 |
A61K38/21 |
代理机构 |
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代理人 |
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地址 |
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