| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method suitable for bulk production of peptide in an industrial scale in recombinant production of an antifusogenic peptide in high yield through an inclusion body process. <P>SOLUTION: The process for the recombinant production of the antifusogenic peptide by expression of a nucleic acid encoding the antifusogenic peptide as repeat peptide in a microbial host cell, isolation of inclusion bodies containing the repeat peptide, solubilization of the inclusion bodies, and isolation of the antifusogenic peptide after cleavage, characterized by washing the inclusion bodies with a denaturing agent at a pH value of or below pH 6.5, solubilizing the inclusion bodies containing the repeat peptide at a pH value of at least pH 9 and cleaving the repeat peptide to obtain the antifusogenic peptide. <P>COPYRIGHT: (C)2004,JPO |
