发明名称 Mutants of enzymes and methods for their use
摘要 Mutants of leucine dehydrogenase sequences, formate dehydrogenase sequences and galactose oxidase sequences are provided. An amino acid sequence that is a mutant of a leucine dehydrogenase sequence as described in SEQ ID 2, or its substantial equivalent, contains at least one mutation selected from the group consisting of F102S, V33A, S351T, N145S and like mutations in subsantially equivalent sequences. An amino acid sequence that is a mutant of a formate dehydrogenase sequence as described in SEQ ID 1, or its substantial equivalent, contains at least one mutation selected from the group consisting of D195S, Y196H, K356T and like mutations in subsantially equivalent sequences. An amino acid sequence that is a mutant of a galactose oxidase sequence as described in SEQ ID 3, or its substantial equivalent, contains at least one mutation selected from the group consisting of N25Y, T94A, D216N, R217C, M278T, Y329C, Q406R, Q406L, V492A, V494A, N521S, N535D, T5491, S567T, T578S and like mutations in subsantially equivalent sequences. Deoxyribonucleic acid molecules containing DNA sequences encoding these mutants are also provided.
申请公布号 US2004115691(A1) 申请公布日期 2004.06.17
申请号 US20030617998 申请日期 2003.07.10
申请人 ROZZELL J. DAVID;HUA LING;MAYHEW MARTIN;NOVICK SCOTT 发明人 ROZZELL J. DAVID;HUA LING;MAYHEW MARTIN;NOVICK SCOTT
分类号 C07H21/04;C12N9/02;C12N9/04;C12N9/06;(IPC1-7):C12Q1/68 主分类号 C07H21/04
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