Method for refolding of proteins

摘要

Method of refolding proteins from a suspension comprising the proteins in a predominantly misfolded form. The method involves adding of a denaturant to the suspension comprising the misfolded proteins to obtain the proteins in a substantially unfolded form. The suspension comprising the unfolded proteins is diluted so as to obtain a mixture where at least part of the proteins are refolded. Subsequently, the refolded proteins can be separated.