| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for testing the activity of a material which may be effective for inhibiting enzyme activity of phospholipase A2. <P>SOLUTION: The method for testing the enzyme activity of phospholipase A2 comprises (a) and (b): (a) the material which may be effective for inhibiting the enzyme activity of phospholipase A2, preferably I type or II type of phospholipase A2 is put in the presence of a substrate which is a phospholipid containing at least one species of ester type fatty acid, preferably the fatty acid having a 15-22C long chain, more preferably an unsaturated or polyunsaturated fatty acid, and releases at least one kind of the fatty acids when it is hydrolyzed; (b) the presence of the fatty acid is detected and the quantity of the same is detected as the case may be. Especially, the method for testing can identify and select effective ingredients which may inhibit the enzyme activity of phospholipase A2. <P>COPYRIGHT: (C)2004,JPO |
