发明名称 |
System and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms |
摘要 |
A system and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms. The method includes processing a plurality of information obtained from a base calling system and creating a plurality of refined base calls using a plurality of original base calls and a plurality of intrinsic peak characteristics. A quality value is also assigned to each of the plurality of refined base calls using the plurality of intrinsic peak characteristics. Processing comprises detecting a plurality of peaks, expanding the plurality of peaks, and resolving the plurality of expanded peaks. Resolving may include fitting the plurality of expanded peaks using a model of a peak shape. A peak resolution parameter is calculated and used in processing. The system may also be trained.
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申请公布号 |
US2004117130(A1) |
申请公布日期 |
2004.06.17 |
申请号 |
US20030727712 |
申请日期 |
2003.12.04 |
申请人 |
PARACEL, INC. |
发明人 |
DENISOV GENNADY A.;AREHART ALAN B.;CURTIN MICHAEL D. |
分类号 |
C12Q1/68;G01N33/48;G01N33/50;G06F19/00;G11C17/00;(IPC1-7):G06F19/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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地址 |
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