| 摘要 |
The invention relates to a method for testing samples containing prion protein for the possible presence of the PrP<Sc >form, according to which: (Step a) the sample is mixed with protease in order to digest protease-sensitive proteins or protein regions; (Step b) after digestion, it is tested whether the sample contains the region PrP 27-30 of the prion protein, which is resistant in the PrP<Sc >form of the prion protein protease, and the presence of PrP<Sc >in the sample is established based on the positive detection of PrP 27-30. The inventive method is characterized in that, during Step b, the sample is additionally tested in order to determine whether a complete digestion of the protease-sensitive region of the prion protein has occurred.
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