| 摘要 |
<p>It is intended to provide a method of synthesizing a protein at a high synthesis efficiency by which a translation reaction can be continued for a long time in a cell-free protein synthesis system, which is applicable to a large-scaled reaction system and which can be carried out by using a simple apparatus. It is also intended to provide a method of performing the synthesis and purification of a protein in a single container after the completion of the synthesis reaction as described above. Moreover, it is intended to provide a reagent kit and an apparatus for carrying out these methods. Namely, a method of producing a target protein with the use of a cell-free protein synthesis system characterized in that the protein is synthesized by bringing a liquid mixture for a transcription/translation reaction or a translation reaction into contact, via an interface, with a gel or a sol containing a substance to be consumed in the transcription/translation reaction or the translation reaction in both of its matrix and its exclusion volume.</p> |
