发明名称 |
EXPRESSION VECTORS ENCODING EPITOPES OF TARGET-ASSOCIATED ANTIGENS AND METHODS FOR THEIR DESIGN |
摘要 |
<p>The invention disclosed herein is directed to methods of identifying a polypeptide suitable for epitope liberation including, for example, the steps of identifying an epitope of interest; providing a substrate polypeptide sequence including the epitope, wherein the substrate polypeptide permits processing by a proteasome; contacting the substrate polypeptide with a composition including the proteasome, under conditions that support processing of the substrate polypeptide by the proteasome; and assaying for liberation of the epitope. The invention further relates to vectors including a housekeeping epitope expression cassette. The housekeeping epitope(s) can be derived from a target-associated antigen, and the housekeeping epitope can be liberatable, that is capable of liberation, from a translation product of the cassette by immunoproteasome processing. The invention also relates to a method of activating a T cell comprising contacting a substrate polypeptide with an APC and contacting the APC with a T cell.</p> |
申请公布号 |
WO03063770(A9) |
申请公布日期 |
2004.06.17 |
申请号 |
WO2002US36098 |
申请日期 |
2002.11.07 |
申请人 |
MANKIND CORPORATION |
发明人 |
SIMARD, JOHN, J.L.;DIAMOND, DAVID, C.;QUI, ZHIYONG;LEI, XIANG-DONG |
分类号 |
A61K39/00;A61K39/12;A61P31/00;A61P35/00;C07K14/47;C12N15/85;G01N33/68;(IPC1-7):C12Q1/00;C12N5/06;C12N15/63;C07H21/04;C12Q1/68 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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