发明名称 |
METHOD FOR PURIFICATION OF VIRAL VECTORS HAVING PROTEINS WHICH BIND SIALIC ACID |
摘要 |
<p>A method for isolating, from a mixture, a virus having a surface protein with a binding site for sialic acid is provided. The method involves contacting the mixture with mucin which has been linked to a solid support and washing the solid support to remove material from the mixture is non-specifically bound to the mucin-linked support. Thereafter, the specifically bound virus (e.g., AAV4 or AAV5) may be removed in a further washing step utilizing a concentrated slat or solution with low pH. Also described are pharmaceutical kits containing solid supports linked to mucin for use in isolating virus having a surface protein with a binding site for sialic acid, or detecting the presence of the virus in a biological sample.</p> |
申请公布号 |
EP1427835(A1) |
申请公布日期 |
2004.06.16 |
申请号 |
EP20020786355 |
申请日期 |
2002.08.06 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
WILSON, JAMES, M.;AURICCHIO, ALBERTO;HILDINGER, MARKUS |
分类号 |
C07K14/005;C12N15/09;C07K1/22;C07K14/015;C07K14/075;C12N7/00;C12N7/01;C12N7/02;C12N15/86;C12N15/864;(IPC1-7):C12N15/861;C12N15/63;C12P1/04 |
主分类号 |
C07K14/005 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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