METHOD OF EFFICIENT RECOMBINATION OF ORF ENCODED IMETHOD OF EFFICIENT RECOMBINATION OF ORF ENCODED IN CDNA, AND TEMPLATE VECTOR, TRAP VECTOR AND PRIMEN CDNA, AND TEMPLATE VECTOR, TRAP VECTOR AND PRIMER USED THERETO R USED THERETO

摘要

-27- The object of the present invention is to solve the problems in excising a D NA region (or gene) with conventional restriction enzymes, or in amplification of the DNA region by PCR. The present invention provides a method for precisely, quickly and simply cloning a target ORF, for example the target ORF encoding a protein, particularly th e target ORF encoding a long-chain ORF comprising bases as many as several thousand by in cDNA, and means such as various vectors used for the cloning method.