摘要 |
<P>PROBLEM TO BE SOLVED: To perform accurate quantitative fluorescence measurement. <P>SOLUTION: This electrophoretic system is constituted of a platform for holding electrophoretic gel, a light source 1803 for illuminating the electrophoretic gel, a first lens assembly 1807 for imaging radiation from a labeled region of the electrophoretic gel on a first detector 1809, a mirror 1811 for reflecting at least a part of illumination from the light source 1803 through a second lens assembly 1815, separately from the first lens assembly 1807, and a processor connected to the first and second detectors. The mirror 1811 determines a light source profile by measuring an intensity pattern of the illumination from the light source 1803 passing through the second lens assembly 1815 by the second detector 1817. The processor normalizes the electrophoretic gel image by using the light source profile. <P>COPYRIGHT: (C)2004,JPO |