MANUFACTURING METHOD AND READOUT SYSTEM FOR BIOPOLYMER ARRAYS

摘要

A method for manufacturing an array of biomolecules that have complementary binding species is disclosed. The method utilizes a master substrate containing a desired master array of binding molecules or ligands, e.g., DNA target molecules. A mixture of complementary, antiligand molecules, e.g., DNA probe molecules, are then hybridized to the ligands of the master array, forming an array of antiligand molecules reversibly bound to the molecules in the master array. The antiligand array is then transferred and bound to a separate substrate, to form a desired array of antiligand molecules on the separate substrate. The process may be repeated to produce multiple arrays or multiple generations of arrays. Also disclosed is a sensor array device and method. The device includes an array of vibratory tympanic elements, each having a specific analyte binding probe carried on the vibratory membrane of the element. A binding event is detected by a change in the vibrational resonance of a selected element.