ANTIBODY AGAINST LESION TISSUE

摘要

It is intended to provide a method of isolating a polynucleotide encoding an antibody against a lesion which involves the following steps: (a) the step of isolating B cells infiltrating into the target lesion; and (b) the step of obtaining a polynucleotide encoding an antibody from the thus isolated B cells. As examples of the lesion, cancer tissues and so on can be cited. Thus, an antibody gene can be obtained without resort to cloning B cells. As a result, it also becomes possible to obtain a gene encoding a human-origin antibody wherein cloning can be hardly effected. By using a cancer tissue as the lesion, a gene of an antibody against cancer can be obtained.