Method of screening for agents that regulate the shedding of membrane bound proteins and methods of use

摘要

The present invention provides a method of screening for agents which may regulate or inhibit the activities of TACE and TGF-alpha. These agents may act by enhancing or inhibiting the activity of DGK-delta. An activity of DGK-delta is the enzymatic conversion of DAG to PA. The method includes contacting a cell or organism with a test agent determining the activity of DGK-delta within the cell. An agent with reduces or increases the activity of DGK may be used to regulate or inhibit the activity of TACE and TGF-alpha. Such agents can be used to treat diseases such as cancer where cell growth and division and inflamation are important factors.