METHOD OF DETECTING TARGET BASE SEQUENCE OF RNA INTERFERENCE, METHOD OF DESIGNING POLYNUCLEOTIDE BASE SEQUENCE CAUSING RNA INTERFERENCE,METHOD OF CONSTRUCTING DOUBLE-STRANDED POLYNUCLEOTIDE, METHOD OF REGULATINGGENE EXPRESSION, BASE SEQUENCE PROCESSING APPARATUS, PROGRAM FOR RUNNING BASE SEQUENCE PROCESSING METHOD ON COMPUTER

摘要

From the base sequence of a target gene of RNA interference, sequence parts fulfilling the following requirements are detected. Based on the results of the detection, an siRNA causing RNAi is designed, synthesized and so on. (a) The base at the 3~-end is adenine, thymine or uracil. (b) The base at the 5~ - end is guanine or cytosine. (c) The base sequence having 7 bases at the 3~-e nd is rich in one or more bases selected from the group consisting of adenine, thymine and uracil. (d) There are bases in such a number as causing RNA interference without showing any cytotoxicity.