| 摘要 |
PURPOSE: A probe for detecting Treponema pallidum, and a kit and method for detecting Treponema pallidum using the same are provided, thereby specifically and sensitively detecting Treponema pallidum and Treponema tpr(Treponema pallidum repeat) subfamily at one time. CONSTITUTION: The probe for detecting Treponema pallidum is selected from oligonucleotides having the nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 5, and complementary oligonucleotides thereof, and complementarily binds with the nucleic acid of Treponema pallidum. A DNA chip for detecting Treponema pallidum comprises the probe for detecting Treponema pallidum, consisting of oligonucleotides containing a portion of the tpr gene of Treponema pallidum. The kit for detecting Treponema pallidum comprises (a) the DNA chip for detecting Treponema pallidum; (b) a primer for PCR amplification of the sample DNA of Treponema pallidum; and (c) a labeling marker for detecting DNA hybridized with the DNA chip, wherein the labeling marker is selected from Cy5, Cy3, biotin-binding material, EDANS (5-(2'-aminoethyl)amino-1-naphthalene sulfuric acid), tetramethyl-rhodamine(TMR), tetramethyl-rhodamine isocyanate(TMRITC), x-rhodamine and texas red. The method for detecting Treponema pallidum comprises the steps of: (a) PCR amplifying the sample DNA of Treponema pallidum using the primer; (b) hybridizing the amplified DNA with the DNA chip; and detecting products hybridized with the probe.
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