METHOD FOR DETECTING PROTEIN COUPLED IN SOLID FACE USING LOCAL DIFFERENTIAL OF COMPLEX REFLECTION COEFFICIENT RATE

摘要

PURPOSE: A method for detecting protein coupled in solid face using local differential of complex reflection coefficient rate is provided, thereby reducing the detection time, simplifying the detection process, requiring no skilled person, and reducing the detection cost by removing a protein marker. CONSTITUTION: The method for detecting protein coupled in solid face using local differential of complex reflection coefficient rate comprises the steps of: passing light(1) through a polarizer(2) and a compensator(3) to polarize the light; irradiating the polarized light to the sample surface of the protein coupled thin layer in solid face; detecting optical intensity of the incident and reflected light from the sample surface using an analyzer(7) and a light sensor; and calculating the complex reflection coefficient rate from the detected optical intensity using equation 1 and 2, wherein N is complex refraction rate; n is refraction rate; k is extinction coefficient; rho(ρ) is complex reflection coefficient ratio; rp is reflection coefficient of p wave; rs is reflection coefficient of s wave; δp(deltap) is phase change of p wave before and after reflection; δs(deltas) is phase change of s wave before and after reflection; and the CCD camera may be used for detecting protein coupled in solid face.