RECOMBINANT PORCINE ADENOVIRUS TYPE 3 EXPRESSING JAPANESE ENCEPHALITIS VIRUS GENE PRME AND USE THEREOF

摘要

PURPOSE: Recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME and use thereof are provided, thereby inducing immunity to Japanese encephalitis virus in human, and improving stability of the vaccine by using adenovirus. CONSTITUTION: A recombinant vector of porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME, pPAV3E3-prME(KCTC 10376BP), is prepared by removing E3 gene of porcine adenovirus type 3, and inserting the Japanese encephalitis virus gene prME under the control of E3 promoter, wherein the Japanese encephalitis virus gene prME has the nucleotide sequence set forth in SEQ ID NO: 1. A host cell transformed with the recombinant vector pPAV3E3-prME(KCTC 10376BP) is provided. The recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME(PAV3E3-prME) is obtained by culturing the transformed host cell. A vaccine for Japanese encephalitis virus comprises the recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME.