发明名称 |
NEW USES OF PROTEINS ENCODED BY BLE GENES AND ANTIBIOTICS FROM THE BLEOMYCIN FAMILY |
摘要 |
The present invention provides use of a protein conjugate comprising a "ble" protein, which has specific binding properties. The protein conjugates are capable of binding reversibly to an antibiotic from the bleomycin family, which property is exploited in a variety of immobilisation methods. In preferred aspects of the invention, the conjugates are used as markers for protein expression and/or folding, or for affinity tagging. The present invention also provides a probe comprising an array of an immobilised antibody from the bleomycin family, which acts as an analyte capture moiety. In another aspect, a purification media is provided, which comprises an antibiotic of the bleomycin family as an analyte capture moiety. Also provided is a method for generating soluble forms of an insoluble protein by expressing the protein as a "ble" fusion protein and selecting in the presence of an antibiotic from the bleomycin family. In a further aspect, the "ble" protein is expressed as a fusion protein in a cell into which is introduced a labelled antibiotic of the bleomycin family, thereby allowing identification of the cellular localisation of the protein. |
申请公布号 |
WO2004046730(A2) |
申请公布日期 |
2004.06.03 |
申请号 |
WO2003IB05430 |
申请日期 |
2003.10.27 |
申请人 |
SENSE THERAPEUTIC LIMITED;HART, DARREN;GODBER, BEN;BLACKBURN, JONATHAN, M.;MCANDREWS, MIKE |
发明人 |
HART, DARREN;GODBER, BEN;BLACKBURN, JONATHAN, M.;MCANDREWS, MIKE |
分类号 |
A61K47/48;C07K14/00;G01N33/58;G01N33/94 |
主分类号 |
A61K47/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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