发明名称 |
METHOD FOR THE RECOMBINATION OF GENETIC ELEMENTS |
摘要 |
A method for the recombination of a gene is disclosed. The method involves the design of unpaired forward and reverse primers having homology to the 5' end of one template and to the 3 end of another template. Short primer extension periods results in a recombined template having paired 5' and 3' ends that can then be amplified. The amplified sample is devoid of any parental template. |
申请公布号 |
WO03072743(A3) |
申请公布日期 |
2004.06.03 |
申请号 |
WO2003US05708 |
申请日期 |
2003.02.26 |
申请人 |
E.I. DU PONT DE NEMOURS AND COMPANY;MILANO, JOSEPH;TANG, XIAO-SONG |
发明人 |
MILANO, JOSEPH;TANG, XIAO-SONG |
分类号 |
C12N15/09;C12N9/00;C12N9/88;C12N15/10;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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