NON-COMPETITIVE IMMUNOASSAY FOR SMALL ANALYTES

摘要

The invention is directed to a non-competitive immunoassay for small analytes, wherein the analyte is reacted with two binding partners. The first binding partner binds to the analyte to form a complex between the first binding partner and the analyte, and the second binding partner binds to the complex formed by the first binding partner and the analyte. The resulting complex formed between the analyte and the binding partners is detected. The binding partners are proteins, such as antibodies including antibody fragments. The invention further relates to reagent pairs and test kits useful in the assays, as well as to the use of the reagent pairs and to a process for their preparation. Novel reagents and means for their preparation are also provided.