METHOD OF PREPARATION OF OLIGOSACCHARIDES

摘要

The invention provides a method of cleaving an 0-linked oligosaccharide from a glycoprotein. The method comprises the steps of contacting a composition comprising a glycoprotein, wherein the glycoprotein comprises 0-linked oligosaccharides, with a solution comprising a borane-ammonia complex to form a mixture comprising the glycoprotein and the borane-ammonia complex, incubating the mixture for a period of time sufficient to cleave the linked oligosaccharides from the glycoprotein, and forming a mixture comprising oligosaccharide alditol products and deglycosylated protein by-products.