发明名称 |
SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHODS FOR FABRICATING THEREOF |
摘要 |
The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties. |
申请公布号 |
WO2004046725(A2) |
申请公布日期 |
2004.06.03 |
申请号 |
WO2003US35258 |
申请日期 |
2003.11.05 |
申请人 |
CORNING INCORPORATED |
发明人 |
FANG, YE;LAHIRI, JOYDEEP;FRUTOS, ANTHONY, G. |
分类号 |
B01J19/00;C40B60/14;G01N33/543 |
主分类号 |
B01J19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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