| 摘要 |
The invention relates to intracellular single domain immunoglobulins, and to a method for determining the ability of a immunoglobulin single domain to bind to a target in an intracellular environment, comprising the steps of: a) providing a first molecule and a second molecule, wherein stable interaction of the first and second molecules leads to the generation of a signal; b) providing a single intracellular immunoglobulin domain which is associated with the first molecule, said single immunoglobulin domain being free of complementary immunoglobulin domains; c) providing an intracellular target which is associated with the second molecule, such that association of the immunoglobulin domain and the target leads to stable interaction of the firs t and second molecules and generation of the signal; and d) assessing the intracellular interaction between the immunoglobulin domain and the target b y monitoring the signal.
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