发明名称 |
Identification of drugs and drug targets by detection of the stress response |
摘要 |
The invention features methods of high throughput screening of candidate drug agents and rapid identification of drug targets by examining induction of the stress response in a host cell, e.g., the stress response in wildtype host cells and/or in host cells that differ in target gene product dosage (e.g., host cells that have two copies of a drug target gene product-encoding sequence relative to one copy). In general, induction of the stress response in wildtype host cells indicates that a candidate agent has activity of the drug. Induction of a relatively lower or undetectable stress response in a host cell comprising an alteration in gene product dosage indicates that the host cell is drug-sensitive and is altered in a gene product that plays a role in resistance to the drug.
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申请公布号 |
US6743583(B2) |
申请公布日期 |
2004.06.01 |
申请号 |
US20010898745 |
申请日期 |
2001.07.03 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
DAVIS RONALD W.;GIAEVER GURI N. |
分类号 |
C12Q1/02;C12Q1/18;C12Q1/68;(IPC1-7):C12Q1/68;G01N33/53;G01N33/567;C12N1/20;C12N1/14;C12N1/16;C12N1/18 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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