IMMORTALIZED HUMAN SKIN CELL LINES, METHOD FOR PREPARING SUCH CELL LINES AND ASSAY USING THE IMMORTALIZED CELLS OF SAID CELL LINES

摘要

The present invention relates to improved continuous (immortalized) cell lines, in particular keratinocytes and melanocytes derived from normal human skin tissue. The present invention also relates to novel serum-free media for isolating, producing and maintaining said improved continuous keratinocyte and melanocyte cell lines. The present invention also relates to methods for producing primary melanocytes and keratinocytes under serum-free conditions without any feeder cells.