MET/FRET BASED METHOD OF TARGET NUCLEIC ACID DETECTION WHEREBY THE DONOR/ACCEPTOR MOIETIES ARE ON COMPLEMENTARY STRANDS

摘要

Disclosure of a method for the detection and quantitation of polynucleotide sequences in a sample of biological or non-biological material through target poly nucleotide sequence amplification whereby MET/FRET occurs between a donor moiety and an acceptor moiety provided separately on at least two separate oligonucleotides that are part of the opposite complementary strands of a nucleic acid segment. .