Expressing mammalian protein complexes in fish

摘要

A fish cell expressing a mammalian protein complex having two peptide species. The cell has a first nucleic acid sequence encoding a first peptide species of the mammalian protein complex and a second nucleic acid sequence encoding a second peptide species of the mammalian protein complex. A multiplicity of peptides of the first peptide species and the second peptide species are expressed and at least one peptide of the first peptide species and at least one peptide of the second peptide species assembly to form a functional mammalian protein complex. The invention also discloses a method of making such a cell, and a cultured fish cell infected with a virus or its progeny for producing the virus.