发明名称 |
HIGH THROUGHPUT AUTOMATIC NUCLEIC ACID ISOLATION AND QUANTITATION METHODS |
摘要 |
A high throughput RNA laboratory protocol is provided for the extraction and maintenance of a sufficient quantity of high quality RNA during sample preparation in order to analyze several genes at a time with assistance of computer analysis. The subject invention includes a method for analyzing RNA comprising the steps of extracting RNA from a complex biological construct i n sufficient quantities to provide accurate RNA data, transferring RNA to an apparatus that maintains the RNA and necessary reagents at a temperature of between about 0 to 10~ C, and analyzing RNA with a computer generated mathematical analysis of the data to access the presence of RNA and ultimate ly test the efficacy of a drug. |
申请公布号 |
CA2502822(A1) |
申请公布日期 |
2004.05.27 |
申请号 |
CA20032502822 |
申请日期 |
2003.11.07 |
申请人 |
PHARMACIA CORPORATION |
发明人 |
DE CIECHI, PAMELA;MCWILLIAMS, DIANA R.;BECKER, ROBERT G.;BOLTEN, CHARLES W.;MILLER, SHAWN D.;SURRY, JEFFREY V.;PINZ, ANDREW T. |
分类号 |
C12N15/10;C12Q1/68;G01N33/48;G01N33/50;G06F19/00;(IPC1-7):C12Q1/68;G06F17/10;G06F17/00 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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