Replicative in vivo gene targeting

摘要

In some embodiments, the invention provides gene targeting systems that renew or regenerate a gene targeting cassette by various mechanisms of DNA replication to enable repeated cycles of gene targeting substrate production in vivo. In some embodiments, successive rounds of gene targeting cassette replication may allow the accumulation of multiple molecules of gene targeting substrate per cell or nucleus, so that the presence of more gene targeting substrate may result in a higher frequency of gene targeting events to produce heritable changes in a target host sequence by processes such as nucleic acid recombination and/or repair.