| 摘要 |
PURPOSE: Provided is a detection method of resistance gene against porcine epidemic diarrhea by examining resistance and susceptibility against Escherichia coli F18 receptor. CONSTITUTION: The detection method is characterized by the steps of: preparing primers for detecting mutation of FUT1 gene; and detecting a pig, which shows resistance against porcine epidemic diarrhea, by PCR using the prepared primers and a test sample collected from a pig. Wherein, the primers are selected from Int 1(SEQ ID NO:1), Int 2(SEQ ID NO:2), Ext 1(SEQ ID NO:3), Ext 2(SEQ ID NO:4) and Ext 2-2(SEQ ID NO:5); and PCR products are subjected to agarose gel electrophoresis.
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