| 摘要 |
PURPOSE: A method for producing L-tryptophan is provided, thereby improving the production yield of L-tryptophan by increasing the amount of phosphoribosylpyrophosphate(PRPP) synthase within a cell. CONSTITUTION: The method for producing L-tryptophan comprises the steps of: constructing a plasmid pTR5(KFCC-10929) containing both of gene trpED and gene prs encoding PRPP synthase by recombination of a plasmid pRS1(KFCC-10930) and a plasmid pLH35; transforming a microorganism with the plasmid pTR5(KFCC-10929) to produce a transformant, Escherichia coli CA15; and culturing Escherichia coli CA15 in a medium containing glucose under conditions of 30 to 37 deg. C, 200 to 300 rpm and pH 6.0 to 8.0 to accumulate L-tryptophan in the cultured medium, wherein the medium contains 75 g/l of glucose, 5 g/l of yeast extract, 2 g/l of KH2PO4, 1 g/l of MgSO4.7H2O, 20 g/l of (NH4)2SO4, 5 g/l of sodium citrate, 1 g/l of NaCl, 0.1 g/l of tyrosine and 40 g/l of CaCO3.
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