| 摘要 |
Disclosed is a new methodology for detecting and/or quantitating nucleic acid sequences, termed "Reporter Oligo Capturing After Specific Hybridization," (ROCASH). Prior art methods are forced to trade sensitivity in the detection assay to achieve increased specificity of hybridization since the sensitivity and specificity are both determined by the same sequence (such as a nucleic acid sequence). In the present method method, unlike the prior art methods, the specificity of hybridization and the sensitivity of the detection step are provided by different complementary binding elements reacting in different steps of the method. Therefore, conditions for the specificity and sensitivity parameters may be optimized independently of each other. |
