Method for monitoring biotechnological processes

摘要

The present invention relates to a method for monitoring biotechnological processes involving microorganisms, said method being characterized in that both the process medium and the microorganisms are directly spectroscoped in said medium by means of attenuated total reflection (ATR) at an ATR crystal whereby microorganisms come at least to a distance from the ATR crystal which corresponds to the evanescent field's depth of penetration, IR spectroscopy being carried out during this time interval, thereupon the microorganisms-containing medium being removed from the ATR crystal which then is washed and its contact is optionally renewed with the microorganisms-containing process medium, and moreover the invention relates to apparatus with which to carry out said method.