The present invention provides novel pGA constructs which are useful as vectors for the delivery of DNA vaccine inserts. The vaccine inserts can include the DNA transcripts of various virus, bacteria, parasite and/or fungi. Also described are methods of raising multi-epitope CD8 T-cell responses by administering therapeutically effective amounts of the novel pGA constructs comprising pathogen vaccine inserts followed by booster immunizations with a live vectored vaccine comprising the same vaccine inserts.
