发明名称 |
Mutant ilvH gene and method for producing L-valine |
摘要 |
The present invention provides an isolated DNA molecule encoding a small subunit of acetohydroxy acid synthase isozyme III originating from Escherichia coli and mutants of Escherichia coli acetohydroxy acid synthase isozyme III, which are free from inhibition by L-valine an can catalyze the conversion of: (a) pyruvate to alpha-acetolactate and (b) alpha-ketobutyrate and pyruvate to alpha-aceto-a-hydroxybutyrate. The present invention also provides methods for producing L-valine by fermentation of a bacterium harboring the novel DNA molecule and/or expressing the mutant acetohydroxy acid synthase isozyme III.
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申请公布号 |
US6737255(B2) |
申请公布日期 |
2004.05.18 |
申请号 |
US20010761782 |
申请日期 |
2001.01.18 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
LIVSHITS VITALIY ARKADYEVICH;DOROSHENKO VERA GEORGIEVNA;GORSHKOVA NATALIYA VASILYEVNA;BELARYEVA ALLA VALENTINOVNA;IVANOVSKAYA LIRINA VALERYEVNA;KHOURGES EVGENI MOISEEVICH;AKHVERDIAN VALERY ZAVENOVICH;GUSYATINER MIKHAIL MARKOVICH;KOZLOV YURY IVANOVICH |
分类号 |
C12N15/09;C12N1/21;C12N9/00;C12N9/88;C12N15/31;C12N15/52;C12N15/70;C12P13/08;C12R1/19;(IPC1-7):C12P13/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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