| 摘要 |
<p>A stable, orally administrable poliomyelitis vaccine is made by mixing a liquid suspension of an attenuated non-pathogenic poliomyelitis virus with comminuted non-hydrated gelatine having a particle size within the range of about 10 to 140 (preferably 60 to 100) mesh (U.S. Standard Sieve). The ingredients are mixed at a temperature between -5 DEG C. and 20 DEG C. (preferbly 3 DEG C. to 5 DEG C.) whereby the gelatine particles become hydrated and absorb the virus forming a free flowing granular vehicle. The virus may be suspended in its propagation medium and the gelatine added thereto with agitation. Anti-fugal agents for example glycerine, corbitol, mannitol, duleitol, nystatik, methyl and propyl esters of p-hydroxybenzoic acid, b -naphthol and mixtures thereof; anti-bacterial agents, for example, penicillin, streptomycin, chloretitracycline, tetracycline and mixtures thereof; excipients, for example, corn starch, potato starch, milo starch, cotton seed meal, soya bean meal, and corn starch containing from 1 to 2% mineral oil may be added. The virus and the propogation media used may be of any known type, and the virus suspensions used may titre at 101 to 109 (preferably 103 to 108) virus doses per c.c. The ratio of virus suspension to gelatine may be from 1:10 to 4:1 preferably about 1 to 2 parts of gelatine to 3 parts by volume of virus suspension. The vaccines are stated to be free-flowing, particulate, granular compositions suitable for encapsulation in, for example, gelatine capsules.</p> |
